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Deletion mutants obtained from the pcDNA 3M plasmid
| Mutant | Reverse-sense primer (5′-3′) | Deletion |
|---|---|---|
| MΔ253-262 | CCGGGCCCCTAATTATCAGTTCTTGCCTC | 253 |
| MΔ237-262 | CCGGGCCCTTATACATAGTAAGCCCATCC | 237 |
| MΔ218-262 | CCGGGCCCCTAGACAATAGTCCTGCTAGG | 218 |
| MΔ146-262 | CCGGGCCCCATCAAGACTTAGTCCTTCTGTACAAC | 146 |
