Table 1. IC50 Values for Seven Reference Compounds Obtained by Four Different Techniques: RapidFire-MS, DESI-MS, Radioassay,3 and LC-MS3.
| IC50 (μM) |
||||
|---|---|---|---|---|
| compound | RapidFire-MS | DESI-MS) | radioassay | LC-MS |
| glafenine | N/Aa | N/Aa | 53.8 ± 14 | 28.1 ± 8.4 |
| zafirlukast | 7.30 ± 1.41 | 2.96 ± 1.99 | 8.57 ± 1.7 | 20.4 ± 10 |
| glyburide | 21.36 ± 6.94 | 3.78 ± 1.83 | 6.79 ± 2.3 | 4.26 ± 0.8 |
| nefazodone | 6.69 ± 1.36 | 4.16 ± 2.76 | 7.11 ± 4.8 | 3.61 ± 0.5 |
| ritonavir | 0.90 ± 0.35 | 0.61 ± 0.54 | 2.92 ± 0.7 | 2.49 ± 0.9 |
| rosiglitazone | 2.26 ± 0.22 | 7.59 ± 3.29 | 5.47 ± 0.5 | 2.60 ± 0.4 |
| cyclosporin A | 5.38 ± 1.46 | 4.49 ± 2.13 | not tested | not tested |
a
N/A means that it was not possible to reliably fit a sigmoidal dose–response curve to determine the IC50 value.