Fig. 2.

The preparation and characterization of self-assembly sphere skin organoids. (A) Microscopic morphology of sphere skin organoids at day1 and day5. Scale bar, 75 μm (B to F) Histology of cross-sectioned organoids harvested at day 7. (B) Hematoxylin/eosin staining illustrated FSO organization. Scale bars, 100 μm and 50 μm. (C) Anti-Ki67 staining indicating cell proliferation within sphere skin organoids. Scale bars, 150 μm and 50 μm. Immunofluorescence staining results of anti-human vimentin (VIM, red, D), anti-human keratin 10/1/14 (K10, K1, and K14, green, E) and anti-human CD31 (red, F) confirmed cell organization into dermal core structures (VIM + CD31) and epidermal layered arrangement (K14). Scale bars, 100 μm and 25 μm. DAPI + nuclei in blue. (B–F) Frames indicating area shown in higher magnification at the right next to overview pictures.