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3D bioprinting skin organoid generate a tightly packed neoepidermal layer with more neovascularization. (A) Samples were taken at day16, and Immunofluorescence staining was performed to test re-epithelialization and neovascularization. (B–E) Immunofluorescence staining of CD31(B) and K14(E) on day 16. CD31, red; CK14, red. Scale bar = 50 μm. Quantitative analysis of (C) CD31 and (D) K14. Data are presented as mean ± SD (n = 3). *P < 0.05, ***P < 0.001 compared with control group.
