Table 1. Cytokine profile after long dsRNA or siRNA treatment.
| Treatment | GM-CSF* | IL-1α | MCP-1 | IL-2 | IL-6 | IL-12p70 | IFN-γ | IL-18 | GRO/KC |
|---|---|---|---|---|---|---|---|---|---|
| Naïve | <24.5 | <24.5 | 173.3 ± 63.8 | <24.5 | <24.5 | <24.5 | <24.5 | 186.3 ± 88.4 | 221.5 ± 49.7 |
| dsControl | <24.5 | <24.5 | 212.0 ± 37.8 | 75.0 ± 67.3 | 32.5 ± 1.1 | <24.5 | 61.3 ± 44.6 | 568.9 ± 476.1 | 224.5 ± 124.2 |
| dsCRF | <24.5 | 58.7 ± 45.6 | 171.3 ± 41.2 | 42.5 ± 12.0 | 300.8 ± 184.2 | 37.2 ± 16.9 | 117.5 ± 62.0 | 114.5 ± 65.0 | 223.8 ± 109.6 |
| dsUcnII | <24.5 | 43.3 ± 25.1 | 155.2 ± 26.1 | <24.5 | 46.2 ± 28.9 | 38.3 ± 18.4 | 90.7 ± 76.6 | 66.0 ± 30.7 | 115.5 ± 45.7 |
| siCRF | <24.5 | 259.4 ± 236.8 | 284.5 ± 61.0 | 46.9 ± 28.3 | 439.3 ± 375.8 | 32.5 ± 9.3 | 131.4 ± 54.6 | 311.3 ± 92.5 | 254.3 ± 49.6 |
| siControl | <24.5 | <24.5 | 212.0 ± 28.7 | 28.8 ± 0.4 | 32.5 ± 10.7 | <24.5 | 25.7 ± 1.6 | 243.3 ± 113.1 | 225.3 ± 52.4 |
Cytokine concentrations in basal plasma sample were measured (pg/ml). Long dsRNA or siRNA treatment had either no effect or a similar effect on cytokine levels (±SEM). si, small interfering; MCP, monocyte chemotatic protein 1; GRO/KC; KC is a chemokine and GROα is the murine homologue.
*
Levels of 8 of 14 cytokines (GM-CSF, IL-1β, IL-4, IL-5, IL-10, IL-12p70, and TNF-α) were within normal (basal) range (<24.5 pg/ml).