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Velocity distribution of gainfi in cells having or lacking myosin II, tracked as in Fig. 2. For reproducible quantitation of tip velocities, the gain was thresholded. High velocities are significantly more abundant in myosin II-null cells (P < 0.1%, Mann–Whitney U test; n = 289 tracked positions in WT cells; n = 446 tracked positions in myosin II-null cells).
