Subject | Omics: Genomics. |
Specific subject area | Argan tree, Mitogenomics |
Type of data | Table, Figure, GenBank, FASTA. |
Data collection | A 9-year-old Argania spinosa specimen, "Amghar," was collected from the Souss Valley plain in Morocco (9°32′00″ N, 30° 24′ 00″ W, 126 m altitude). DNA was extracted from its leaves using the QIAgen Plant DNeasy Mini Kit, and a paired-end library was constructed using the Nextera DNA Library Prep Kit. Sequencing on the Illumina HiSeq XTen platform yielded 77,382,038 reads. Mitochondrial sequences were assembled using GetOrganelle v.1.7.1 with Camellia sinensis as a reference. Protein-coding genes (PCGs), rRNA, and tRNA were annotated using BlastX, BlastN, and tRNAscan-SE, respectively, and manually validated with Geneious Prime. The mitochondrial genome map was visualised with OGDraw 1.3.1. For phylogenetic analysis of Ericales, 7 common PCGs from 15 members were extracted, aligned with Mafft v.7.5.0.8, and analysed with MEGA v.11.0.10 under the best-fit model from Modeltest. |
Data source location | Institution: Faculty of Sciences of Agadir Region: Souss Valley City: Agadir Country: Morocco Latitude: 9°32′00″ N Longitude: 30° 24′ 00″ W |
Data accessibility | Repository name: NCBI Sequence Read Archive (SRA) Data identification number: SRR6062045 Direct URL to data: https://www.ncbi.nlm.nih.gov/sra/SRR6062045\ Repository name: NCBI BioProject Data identification number: PRJNA294096 Direct URL to data: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294096 Repository name: NCBI BioSample Data identification number: SRX3207156 Direct URL to data: https://www.ncbi.nlm.nih.gov/biosample/SAMN04014715 Repository name: NCBI GenBank Data identification number: MZ151883.2 Direct URL to data: https://www.ncbi.nlm.nih.gov/nuccore/MZ151883 |