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. Author manuscript; available in PMC: 2024 Oct 15.
Published in final edited form as: Sci Total Environ. 2024 Jul 2;947:174478. doi: 10.1016/j.scitotenv.2024.174478

Fig. 4.

Fig. 4.

PFOS induces ROS generation and astrocyte activation. Fluorescent images of ROS indicator dye in two-month-old hMOs (a) and the corresponding quantification (b) after a 7-day exposure in control group (vehicle) and PFOS group (300 μM) (mean ± s.e.m., n = 5 organoids, unpaired t-test, from 3 independent experiments). Immunofluorescent staining of astrocytes (GFAP) in two-month-old hMOs (c), the corresponding quantification (mean ± s.e.m., unpaired t-test, n = 5 organoids, from 3 independent experiments) (d), and qPCR analysis of relative GFAP gene expression (e) after a 7-day exposure in control group (vehicle treated with 0.05 % DMSO) and PFOS group (300 μM) (mean ± s.e.m., unpaired t-test, n = 3 organoids, from 3 independent experiments). Scale bar: 50 μm.