REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Blood from the 79 subjects included in the study from four time points (Days 0, 28, 90 and 180 post-vaccination)	La Paz University Hospital (Madrid), San Carlos Hospital (Madrid), Cruces University Hospital (Vizcaya), Vall d’Hebron University Hospital (Barcelona) and Clinic Hospital (Barcelona)	N/A
Chemicals, peptides, and recombinant proteins
Codon-optimized spike DNA sequences for D614G, B.1.617.2, BA.1, BA.4/BA.5, BQ.1.1, XBB.1.5/XBB.1.9, BA.2.86, and JN.1 SARS-CoV-2 variants	GeneArt Gene Synthesis (ThermoFisher)	N/A
Critical commercial assays
Elecsys® Anti-SARS-CoV-2 S	Roche Diagnostics	Cat#09203079190
BioPlex 2200 SARS-CoV-2 IgG	Bio-Rad Laboratories	Cat# 12014192
Renilla Luciferase Assay	Promega	Cat#E2820
Experimental models: Cell lines
Vero E6	Laboratory of Dr. Antonio Alcamí	N/A
HEK-293T	NIBSC	N/A
Recombinant DNA
pcDNA3.1D/V5-His-TOPO	Invitrogen™	Cat#K490001
pcDNA-VSV-G	Laboratory of Dr. Arenzana Seisdedos	N/A
pNL4-3ΔenvRen	García-Pérez et al., 200731	N/A
pcDNA3.1-S-CoV2Δ19-G614	This paper	N/A
pcDNA3.1-S-CoV2Δ19-Delta	This paper	N/A
pcDNA3.1-S-CoV2Δ19-BA.1	This paper	N/A
pcDNA3.1-S-CoV2Δ19-BA.4-BA.5	This paper	N/A
pcDNA3.1-S-CoV2Δ19-BQ.1.1	This paper	N/A
pcDNA3.1-S-CoV2Δ19-XBB.1.5	This paper	N/A
pcDNA3.1-S-CoV2Δ19-BA.2.86	This paper	N/A
pcDNA3.1-S-CoV2Δ19-JN.1	This paper	N/A
Software and algorithms
GraphPad Prism version 9.0.1	GraphPad Software, Inc.	N/A
R 4.3.2 and ggplot2 package	Free software	N/A