Fig. 1.

Generation of DGKι-deficient animals by homologous recombination. (A) DGKι knockout targeting vector with thymidine kinase (TK) and neomycin-resistance cassettes. EcoRI was used for Southern blotting, and the shaded box represents the probe. Also shown are PCR primers (arrows) used for genotyping and the size of the PCR products. (B) PCR to detect the genotype of the animals using genomic DNA from tail lysates. (C) RT-PCR analysis of mRNA from wild-type (+/+) or knockout (–/–) mouse livers to detect expression of DGKι mRNA.