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. 2001 Feb;75(3):1378–1386. doi: 10.1128/JVI.75.3.1378-1386.2001

FIG. 6.

FIG. 6

HCMV activation of KSHV in HUVEC. (A) Visualization of the nuclear expression of the lytic cycle ORF 59 protein with MAb 11D1 in HUVEC infected with rKSHV.152, minus and plus HCMV. Panel 1, HUVEC infected with rKSHV.152 3 dpi reacted with MAb 11D1 and Alexa 594 (red) and stained with DAPI (blue); panel 2, HUVEC coinfected with rKSHV.152 and HCMV reacted with MAb 11D1 and Alexa 594 (red) and stained with DAPI (blue). (B) Gardella gel analysis of KSHV DNA isolated from HUVEC infected with rKSHV.152, minus and plus HCMV. Lane 1, HUVEC infected with rKSHV.152; lane 2, HUVEC coinfected with rKSHV.152 and HCMV; lane 3, KSHV virion; lane 4, BCBL-1 cells. The positions of linear and circular KSHV genomes are indicated. (C) Presentation of two experiments showing the number of GFP-positive 293 cells resulting from infection with virus harvested from HUVEC cultures infected with rKSHV.152 and coinfected with rKSHV.152 and HCMV.