Fig. 4.

Interaction of DAP10 TM peptides with NKG2D. (A) DAP10 TM peptides formed a dimer, based on two-step PC→ SBP snIP of translation reactions with PC- and SBP-tagged DAP10.TM chains (lane 3). Data are representative of three experiments. (B) The DAP10.TM protein assembled with full-length NKG2D because the complex could be isolated by two-step snIP for the PC and SBP tags attached to DAP10.TM and NKG2D, respectively. *, Mixing controls demonstrated that assembly occurred in ER membranes. Data are representative of two experiments.