FIG. 8.

Quantitative comparison of the motility of Ad5 and Ad7 at different time points. A549 cells were infected with Cy3-Ad5 or Cy3-Ad7 (10¹¹ particles/ml) for 10 min, at 37°C, washed out, and incubated at 37°C for 30 or 240 min. Digital segments of the fluorescent image fields were analyzed with respect to the number of linear translocations (>2 μm) observed. Data are normalized with respect to the number of viruses visible in the field and the duration of observation. The motility of Ad7 at 240 min after infection was increased significantly compared with that 30 min after infection, whereas the motility of Ad5 decreased over the same interval.