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Colocalization of NS5A and endogenous p53 in HepG2 cells. Immunofluorescent staining using a monoclonal antibody to p53 exhibits nuclear localization of p53 in mock-transfected cells (A) or perinuclear localization of p53 in CMV-NS5A-transfected cells (B). Cells transfected with NS5A were stained with a monoclonal antibody to p53 (C) and a rabbit antibody to NS5A (D). Fluorescence images of panels C and D were superimposed digitally for fine comparison (E).
