Table 1.
Staphylococcus aureus strains and plasmids used in this study.
| Strain or Plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Bacterial Strains | ||
| RN4220 | Highly transformable S. aureus | [37] |
| AH1263 | USA300 CA-MRSA strain LAC lacking LAC-p03; wild-type strain used for these studies | [38] |
| JLB15 | AH1263 saeR::Tn, referred to as saeR | [39] |
| JLB24 | AH1263 hla::Tn | [40] |
| JLB110 | AH1263 ΔyjbIH | [8] |
| JLB134 | AH1263 ΔyjbI | [8] |
| JLB145 | AH1263 yjbH::Tn | [8] |
| JLB147 | AH1263 ΔyjbH | [8] |
| JLB323 | AH1263 ΔyjbIH hla::Tn | This study |
| JLB316 | AH1263 ΔRNAIII-agrBDCA | [41] |
| JLB331 | AH1263 ΔsaeRS | [42] |
| JLB362 | RN4220 Phla-lux | This study |
| JLB366 | AH1263 Phla-lux | This study |
| JLB367 | AH1263 ΔyjbIH Phla-lux | This study |
| JLB368 | AH1263 ΔRNAIII-agrBDCA Phla-lux | This study |
| JLB371 | AH1263 ΔsaeRS Phla-lux | This study |
| Plasmids | ||
| pCP4 | yjbIH pKK22-based complementation plasmid, referred to as pyjbIH | [8] |
| pCP5 | yjbH pKK22-based complementation plasmid, referred to as pyjbH | [8] |
| pCP6 | yjbI pKK22-based complementation plasmid, referred to as pyjbI | [8] |
| pCP20/pRKC1032 | Ppsmα-lacZ | [43] |
| pCP25 | hla under control of non-native promoter | This study |
| pJB1017 | Phla-lacZ reporter | [39] |
| pJB1018 | Pcoa-lacZ reporter | [39] |
| pJB1063 | lux system in pJC1112 | This study |
| pJB1066 | Hla lux reporter (Phla-lux) | This study |
| pJC1112 | ErmR plasmid that integrates into SapI1 | [44] |
| pKK22 | TmpR complementation plasmid | [24] |
| pRN7023 | Helper plasmid for SapI1 integration | [45] |
| pHC125 | luxCDABEG chromosomal integration plasmid for φ11 attachment site | [46] |
aTn indicates the insertion of the Bursa aurealis transposon from the Nebraska Transposon Mutant Library.