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. Author manuscript; available in PMC: 2024 Sep 16.
Published in final edited form as: J Vis Exp. 2021 Feb 11;(168):10.3791/62093. doi: 10.3791/62093

Figure 3: JAM-A regulates intestinal permeability in vivo.

Figure 3:

(A) JAM-A deficiency (Jam-a−/−) led to increased colonic permeability to 4 kDa FITC-dextran. Jam-a−/− (13x animals; black dots) were compared with Jam-a+/+ controls (12x animals; white dots). 4 kDa FITC-dextran (1 mg/mL) in HBSS was injected into the pcLoop lumen. Fluorescence was measured in blood serum after a 120 min incubation period. Data are expressed as means ± SEM; n = 3 independent experiments. ****P < 0.0001; Mann-Whitney U test. (B) Increased colonic permeability to 4 kDa FITC-dextran in Villin-cre; Jam-afl/fl (18x animals, black dots) compared to controls (Jam-afl/fl, 12x animals, white dots). Data are means ± SEM; n = 4 independent experiments. ****P < 0.0001; Mann-Whitney U test. This figure has been modified from Flemming S, Luissint AC et al.30.