TABLE 1.
MTAP Immunostaining in Human Tumors
| MTAP immunostaining | |||||||
|---|---|---|---|---|---|---|---|
| Tumor category | Tumor entity | on TMA (n) | Analyzable (n) | 0 (%) | 1+ (%) | 2+ (%) | 3+ (%) |
| Tumors of the skin | Basal cell carcinoma of the skin | 89 | 68 | 2.9 | 45.6 | 38.2 | 13.2 |
| Benign nevus | 29 | 23 | 0.0 | 4.3 | 26.1 | 69.6 | |
| Squamous cell carcinoma of the skin | 145 | 103 | 9.7 | 68.9 | 19.4 | 1.9 | |
| Malignant melanoma | 65 | 51 | 15.7 | 19.6 | 49.0 | 15.7 | |
| Malignant melanoma lymph node metastasis | 86 | 67 | 28.4 | 32.8 | 23.9 | 14.9 | |
| Merkel cell carcinoma | 2 | 1 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Tumors of the head and neck | Squamous cell carcinoma of the larynx | 109 | 81 | 24.7 | 56.8 | 18.5 | 0.0 |
| Squamous cell carcinoma of the pharynx | 60 | 59 | 11.9 | 28.8 | 49.2 | 10.2 | |
| Oral squamous cell carcinoma (floor of the mouth) | 130 | 119 | 11.8 | 44.5 | 37.8 | 5.9 | |
| Pleomorphic adenoma of the parotid gland | 50 | 38 | 0.0 | 60.5 | 36.8 | 2.6 | |
| Warthin tumor of the parotid gland | 104 | 63 | 0.0 | 60.3 | 39.7 | 0.0 | |
| Adenocarcinoma, NOS (papillary cystadenocarcinoma) | 14 | 6 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Salivary duct carcinoma | 15 | 3 | 66.7 | 33.3 | 0.0 | 0.0 | |
| Acinic cell carcinoma of the salivary gland | 181 | 53 | 1.9 | 62.3 | 35.8 | 0.0 | |
| Adenocarcinoma NOS of the salivary gland | 109 | 29 | 10.3 | 65.5 | 20.7 | 3.4 | |
| Adenoid cystic carcinoma of the salivary gland | 180 | 33 | 6.1 | 27.3 | 63.6 | 3.0 | |
| Basal cell adenocarcinoma of the salivary gland | 25 | 12 | 0.0 | 33.3 | 41.7 | 25.0 | |
| Basal cell adenoma of the salivary gland | 101 | 26 | 0.0 | 26.9 | 65.4 | 7.7 | |
| Epithelial-myoepithelial carcinoma of the salivary gland | 53 | 18 | 0.0 | 44.4 | 55.6 | 0.0 | |
| Mucoepidermoid carcinoma of the salivary gland | 343 | 149 | 4.0 | 65.8 | 29.5 | 0.7 | |
| Myoepithelial carcinoma of the salivary gland | 21 | 10 | 0.0 | 70.0 | 30.0 | 0.0 | |
| Myoepithelioma of the salivary gland | 11 | 8 | 0.0 | 37.5 | 62.5 | 0.0 | |
| Oncocytic carcinoma of the salivary gland | 12 | 2 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Polymorphous adenocarcinoma, low grade, of the salivary gland | 41 | 2 | 0.0 | 0.0 | 50.0 | 50.0 | |
| Pleomorphic adenoma of the salivary gland | 53 | 22 | 4.5 | 45.5 | 40.9 | 9.1 | |
| Tumors of the lung, pleura and thymus | Adenocarcinoma of the lung | 196 | 167 | 12.0 | 64.7 | 21.6 | 1.8 |
| Squamous cell carcinoma of the lung | 80 | 59 | 16.9 | 62.7 | 20.3 | 0.0 | |
| Mesothelioma, epithelioid | 40 | 25 | 32.0 | 24.0 | 40.0 | 4.0 | |
| Mesothelioma, biphasic | 29 | 19 | 36.8 | 42.1 | 15.8 | 5.3 | |
| Thymoma | 29 | 21 | 0.0 | 52.4 | 47.6 | 0.0 | |
| Lung, NET | 29 | 28 | 53.6 | 46.4 | 0.0 | 0.0 | |
| Tumors of the female genital tract | Squamous cell carcinoma of the vagina | 78 | 54 | 18.5 | 38.9 | 37.0 | 5.6 |
| Squamous cell carcinoma of the vulva | 157 | 119 | 21.0 | 48.7 | 27.7 | 2.5 | |
| Squamous cell carcinoma of the cervix | 136 | 119 | 1.7 | 57.1 | 35.3 | 5.9 | |
| Adenocarcinoma of the cervix | 23 | 22 | 4.5 | 31.8 | 59.1 | 4.5 | |
| Endometrioid endometrial carcinoma | 338 | 296 | 0.7 | 61.5 | 35.5 | 2.4 | |
| Endometrial serous carcinoma | 86 | 67 | 1.5 | 38.8 | 41.8 | 17.9 | |
| Carcinosarcoma of the uterus | 57 | 52 | 0.0 | 59.6 | 34.6 | 5.8 | |
| Endometrial carcinoma, high grade, G3 | 13 | 12 | 0.0 | 16.7 | 75.0 | 8.3 | |
| Endometrial clear cell carcinoma | 9 | 7 | 0.0 | 85.7 | 14.3 | 0.0 | |
| Endometrioid carcinoma of the ovary | 130 | 96 | 1.0 | 39.6 | 44.8 | 14.6 | |
| Serous carcinoma of the ovary | 580 | 443 | 1.1 | 38.1 | 47.9 | 12.9 | |
| Mucinous carcinoma of the ovary | 101 | 65 | 29.2 | 44.6 | 18.5 | 7.7 | |
| Clear cell carcinoma of the ovary | 51 | 32 | 6.3 | 62.5 | 31.3 | 0.0 | |
| Carcinosarcoma of the ovary | 47 | 40 | 7.5 | 40.0 | 42.5 | 10.0 | |
| Granulosa cell tumor of the ovary | 44 | 42 | 2.4 | 38.1 | 45.2 | 14.3 | |
| Leydig cell tumor of the ovary | 4 | 4 | 0.0 | 0.0 | 100.0 | 0.0 | |
| Sertoli cell tumor of the ovary | 1 | 1 | 0.0 | 0.0 | 100.0 | 0.0 | |
| Sertoli Leydig cell tumor of the ovary | 3 | 3 | 0.0 | 33.3 | 66.7 | 0.0 | |
| Steroid cell tumor of the ovary | 3 | 3 | 0.0 | 0.0 | 100.0 | 0.0 | |
| Brenner tumor | 41 | 39 | 5.1 | 12.8 | 66.7 | 15.4 | |
| Tumors of the breast | Invasive breast carcinoma of no special type | 499 | 454 | 1.5 | 48.0 | 40.5 | 9.9 |
| Lobular carcinoma of the breast | 192 | 166 | 0.0 | 34.3 | 54.8 | 10.8 | |
| Medullary carcinoma of the breast | 23 | 19 | 5.3 | 21.1 | 42.1 | 31.6 | |
| Tubular carcinoma of the breast | 20 | 11 | 0.0 | 27.3 | 54.5 | 18.2 | |
| Mucinous carcinoma of the breast | 29 | 26 | 0.0 | 26.9 | 65.4 | 7.7 | |
| Phyllodes tumor of the breast | 50 | 47 | 0.0 | 27.7 | 57.4 | 14.9 | |
| Tumors of the digestive system | Adenomatous polyp, low-grade dysplasia | 50 | 42 | 0.0 | 33.3 | 64.3 | 2.4 |
| Adenomatous polyp, high-grade dysplasia | 50 | 48 | 0.0 | 27.1 | 60.4 | 12.5 | |
| Adenocarcinoma of the colon | 2483 | 1892 | 0.8 | 37.3 | 41.8 | 20.1 | |
| Gastric adenocarcinoma, diffuse type | 215 | 151 | 4.0 | 64.9 | 31.1 | 0.0 | |
| Gastric adenocarcinoma, intestinal type | 215 | 168 | 11.9 | 44.0 | 38.7 | 5.4 | |
| Gastric adenocarcinoma, mixed type | 62 | 57 | 5.3 | 57.9 | 29.8 | 7.0 | |
| Adenocarcinoma of the esophagus | 83 | 45 | 13.3 | 46.7 | 28.9 | 11.1 | |
| Squamous cell carcinoma of the esophagus | 76 | 37 | 27.0 | 32.4 | 37.8 | 2.7 | |
| Squamous cell carcinoma of the anal canal | 91 | 78 | 5.1 | 43.6 | 46.2 | 5.1 | |
| Cholangiocarcinoma | 58 | 43 | 16.3 | 51.2 | 32.6 | 0.0 | |
| Gallbladder adenocarcinoma | 51 | 42 | 40.5 | 42.9 | 16.7 | 0.0 | |
| Gallbladder Klatskin tumor | 42 | 34 | 11.8 | 61.8 | 17.6 | 8.8 | |
| HCC | 312 | 294 | 9.2 | 60.9 | 24.8 | 5.1 | |
| Ductal adenocarcinoma of the pancreas | 659 | 506 | 32.4 | 42.1 | 22.3 | 3.2 | |
| Pancreatic/ampullary adenocarcinoma | 98 | 78 | 17.9 | 51.3 | 29.5 | 1.3 | |
| Acinar cell carcinoma of the pancreas | 18 | 16 | 18.8 | 50.0 | 18.8 | 12.5 | |
| GIST | 62 | 55 | 3.6 | 56.4 | 38.2 | 1.8 | |
| Appendix, NET | 25 | 14 | 35.7 | 42.9 | 21.4 | 0.0 | |
| Colorectal, NET | 12 | 10 | 70.0 | 10.0 | 20.0 | 0.0 | |
| Ileum, NET | 53 | 47 | 78.7 | 19.1 | 2.1 | 0.0 | |
| Pancreas, NET | 101 | 89 | 11.2 | 71.9 | 12.4 | 4.5 | |
| Colorectal, NEC | 14 | 13 | 7.7 | 38.5 | 46.2 | 7.7 | |
| Ileum, NEC | 8 | 5 | 80.0 | 0.0 | 20.0 | 0.0 | |
| Gallbladder, NEC | 4 | 4 | 0.0 | 25.0 | 75.0 | 0.0 | |
| Pancreas, NEC | 14 | 12 | 8.3 | 58.3 | 33.3 | 0.0 | |
| Tumors of the urinary system | Noninvasive papillary urothelial carcinoma, pTa G2 low-grade | 87 | 76 | 10.5 | 27.6 | 25.0 | 36.8 |
| Noninvasive papillary urothelial carcinoma, pTa G2 high-grade | 80 | 66 | 28.8 | 16.7 | 45.5 | 9.1 | |
| Noninvasive papillary urothelial carcinoma, pTa G3 | 126 | 101 | 18.8 | 26.7 | 42.6 | 11.9 | |
| Urothelial carcinoma, pT2-4 G3 | 735 | 573 | 29.0 | 30.4 | 28.8 | 11.9 | |
| Squamous cell carcinoma of the bladder | 22 | 21 | 38.1 | 52.4 | 4.8 | 4.8 | |
| Small cell NEC of the bladder | 5 | 4 | 0.0 | 0.0 | 50.0 | 50.0 | |
| Sarcomatoid urothelial carcinoma | 25 | 12 | 8.3 | 33.3 | 50.0 | 8.3 | |
| Urothelial carcinoma of the kidney pelvis | 62 | 49 | 36.7 | 26.5 | 28.6 | 8.2 | |
| Clear cell renal cell carcinoma | 1287 | 1109 | 0.6 | 62.2 | 36.4 | 0.7 | |
| Papillary renal cell carcinoma | 368 | 318 | 1.3 | 45.3 | 47.5 | 6.0 | |
| Clear cell (tubulo) papillary renal cell carcinoma | 26 | 21 | 0.0 | 52.4 | 42.9 | 4.8 | |
| Chromophobe renal cell carcinoma | 170 | 146 | 0.0 | 73.3 | 26.0 | 0.7 | |
| Oncocytoma of the kidney | 257 | 222 | 0.0 | 49.5 | 49.5 | 0.9 | |
| Tumors of the male genital organs | Adenocarcinoma of the prostate, Gleason 3+3 | 83 | 67 | 0.0 | 47.8 | 43.3 | 9.0 |
| Adenocarcinoma of the prostate, Gleason 4+4 | 80 | 65 | 0.0 | 32.3 | 60.0 | 7.7 | |
| Adenocarcinoma of the prostate, Gleason 5+5 | 85 | 70 | 0.0 | 38.6 | 57.1 | 4.3 | |
| Adenocarcinoma of the prostate (recurrence) | 258 | 159 | 1.9 | 42.8 | 49.1 | 6.3 | |
| Small cell NEC of the prostate | 2 | 1 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Seminoma | 682 | 454 | 0.4 | 72.5 | 24.2 | 2.9 | |
| Embryonal carcinoma of the testis | 54 | 44 | 0.0 | 40.9 | 50.0 | 9.1 | |
| Leydig cell tumor of the testis | 31 | 31 | 0.0 | 22.6 | 77.4 | 0.0 | |
| Sertoli cell tumor of the testis | 2 | 2 | 0.0 | 50.0 | 50.0 | 0.0 | |
| Sex cord stromal tumor of the testis | 1 | 1 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Spermatocytic tumor of the testis | 1 | 1 | 0.0 | 0.0 | 100.0 | 0.0 | |
| Yolk sac tumor | 53 | 40 | 0.0 | 62.5 | 30.0 | 7.5 | |
| Teratoma | 53 | 40 | 0.0 | 45.0 | 55.0 | 0.0 | |
| Squamous cell carcinoma of the penis | 92 | 86 | 2.3 | 64.0 | 25.6 | 8.1 | |
| Tumors of endocrine organs | Adenoma of the thyroid gland | 113 | 99 | 0.0 | 58.6 | 39.4 | 2.0 |
| Papillary thyroid carcinoma | 391 | 335 | 0.0 | 41.2 | 57.3 | 1.5 | |
| Follicular thyroid carcinoma | 154 | 104 | 0.0 | 55.8 | 42.3 | 1.9 | |
| Medullary thyroid carcinoma | 111 | 74 | 5.4 | 83.8 | 10.8 | 0.0 | |
| Parathyroid gland adenoma | 43 | 37 | 0.0 | 35.1 | 56.8 | 8.1 | |
| Anaplastic thyroid carcinoma | 45 | 39 | 5.1 | 43.6 | 48.7 | 2.6 | |
| Adrenal cortical adenoma | 48 | 46 | 0.0 | 41.3 | 58.7 | 0.0 | |
| Adrenal cortical carcinoma | 27 | 27 | 0.0 | 59.3 | 33.3 | 7.4 | |
| Pheochromocytoma | 51 | 44 | 2.3 | 86.4 | 11.4 | 0.0 | |
| Paraganglioma | 41 | 36 | 0.0 | 72.2 | 27.8 | 0.0 | |
| Tumors of hematopoietic and lymphoid tissues | Hodgkin lymphoma | 103 | 50 | 50.0 | 46.0 | 4.0 | 0.0 |
| B-SLL, B-cell type (B-CLL) | 50 | 48 | 0.0 | 39.6 | 41.7 | 18.8 | |
| DLBCL | 113 | 108 | 4.6 | 56.5 | 33.3 | 5.6 | |
| Follicular lymphoma | 88 | 86 | 7.0 | 60.5 | 31.4 | 1.2 | |
| T-cell non-Hodgkin lymphoma | 25 | 22 | 13.6 | 50.0 | 36.4 | 0.0 | |
| Mantle cell lymphoma | 18 | 18 | 0.0 | 33.3 | 61.1 | 5.6 | |
| Marginal zone lymphoma | 16 | 14 | 14.3 | 35.7 | 28.6 | 21.4 | |
| DLBCL in the testis | 16 | 14 | 7.1 | 85.7 | 7.1 | 0.0 | |
| Burkitt lymphoma | 5 | 4 | 0.0 | 50.0 | 50.0 | 0.0 | |
| Tumors of soft tissue and bone | Granular cell tumor | 23 | 15 | 0.0 | 0.0 | 93.3 | 6.7 |
| Leiomyoma | 50 | 41 | 0.0 | 90.2 | 9.8 | 0.0 | |
| Leiomyosarcoma | 94 | 70 | 5.7 | 57.1 | 37.1 | 0.0 | |
| Atypical lipomatous tumor/well-differentiated liposarcoma | 18 | 11 | 0.0 | 54.5 | 45.5 | 0.0 | |
| Dedifferentiated liposarcoma | 54 | 42 | 0.0 | 73.8 | 26.2 | 0.0 | |
| Myxoid liposarcoma | 18 | 14 | 0.0 | 64.3 | 35.7 | 0.0 | |
| Pleomorphic liposarcoma | 3 | 1 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Pleomorphic rhabdomyosarcoma | 3 | 1 | 0 | 100 | 0 | 0 | |
| MPNST | 15 | 13 | 30.8 | 53.8 | 15.4 | 0.0 | |
| Myofibrosarcoma | 26 | 24 | 0.0 | 41.7 | 54.2 | 4.2 | |
| Angiosarcoma | 42 | 26 | 0.0 | 50.0 | 46.2 | 3.8 | |
| Angiomyolipoma | 91 | 60 | 0.0 | 85.0 | 15.0 | 0.0 | |
| Dermatofibrosarcoma protuberans | 21 | 16 | 6.3 | 50.0 | 43.8 | 0.0 | |
| Ganglioneuroma | 14 | 12 | 0.0 | 91.7 | 8.3 | 0.0 | |
| Kaposi sarcoma | 8 | 4 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Neurofibroma | 117 | 104 | 0.0 | 90.4 | 9.6 | 0.0 | |
| Sarcoma, NOS | 74 | 63 | 14.3 | 46.0 | 39.7 | 0.0 | |
| Ewing sarcoma | 23 | 10 | 0.0 | 50.0 | 30.0 | 20.0 | |
| Alveolar rhabdomyosarcoma | 2 | 1 | 0.0 | 100.0 | 0.0 | 0.0 | |
| Pleomorphic rhabdomyosarcoma | 2 | 2 | 50.0 | 50.0 | 0.0 | 0.0 | |
| Embryonal rhabdomyosarcoma | 3 | 2 | 0.0 | 0.0 | 50.0 | 50.0 | |
| Schwannoma | 122 | 113 | 0.9 | 59.3 | 38.1 | 1.8 | |
| Synovial sarcoma | 12 | 10 | 0.0 | 10.0 | 30.0 | 60.0 | |
| Osteosarcoma | 19 | 12 | 0.0 | 66.7 | 25.0 | 8.3 | |
| Chondrosarcoma | 15 | 10 | 20.0 | 40.0 | 40.0 | 0.0 | |
| Rhabdoid tumor | 5 | 5 | 0.0 | 40.0 | 60.0 | 0.0 | |
| Solitary fibrous tumor | 17 | 17 | 0.0 | 41.2 | 52.9 | 5.9 | |
Immunostaining score “0” indicates complete loss of MTAP expression.
CLL indicates chronic lymphocytic lymphoma; DLBCL, diffuse large B-cell lymphoma; GIST, gastrointestinal stromal tumor; MPNST, malignant peripheral nerve sheath tumor; NEC, neuroendocrine carcinoma; NOS, not otherwise specified; SLL, small lymphocytic lymphoma.