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. 2005 May 26;102(23):8275–8280. doi: 10.1073/pnas.0408589102

Fig. 2.

Fig. 2.

Susceptibility of PEFs from transgenic mice to HCoV-229E infection. (A) Cells from the indicated transgenic mice lines or from control cells (MRC-5 and ICR WT mice) were infected with HCoV-229E grown in MRC-5 cells at 32°C. Cells were incubated at 32°C, and virus was detected by immunofluorescence using monoclonal antibody 5–11H.6 specific for HCoV-229E S protein. (B) (Upper) Growth of HCoV-229E was monitored by studying the expression of a HCoV-229E S gene mRNA. (Lower) The expression of β-actin mRNA was analyzed as a control. hAPN+/+ and hAPN-/-, transgenic mice homozygous for hAPN or not carrying this transgene; Stat1+/+ and Stat1-/-, WT mice or Stat1-deficient mice; MM, molecular markers in bp.