Fig 1. Genetic transformation of sugarcane spindle leaf roll segments following agro-infectionand semi-quantitative reverse transcription-PCRanalysis of putative plants.

(a) Gene construct showing endochitinase driven by CaMV35S promoter.(b) Pre-cultured leaf roll segments. (c) Direct emergence of roots. (d)Direct emergence of shoots. (e) Multiple shoot formation. (f) Plantlet formation. (g) Hardening of plantlets on moist cotton. (h) Transfer of plants to soil. (i) RNA of PCR positive plants designated as 1–9 to 5–65. (j) cDNA amplified by 26S rRNA gene specific primers. (k)cDNA amplified by endochitinase gene specific primers.