Table 1. Bioassay on transgenic sugarcane plants for red rot incidence.
| Plant designation | Relative endochitinase expression (2- ΔΔCT) |
Pathotype | ||||
|---|---|---|---|---|---|---|
| CF08 | CF13 | |||||
| Scale (0–9)* | Reaction | Scale (0–9)* | Reaction | |||
| NTC | 1 (0.90–1.01) | 8.0 | S | 9.0 | HS | |
| Chit1-9 | 1.83 (1.80–1.87) | 4.2 | MS | 7.6 | S | |
| Chit 1–64 | 3.60 (3.41–3.81) | 4.1 | MS | 8.6 | HS | |
| Chit 2–39 | 4.82 (4.79–4.86) | 3.6 | MR | 8.3 | HS | |
| Chit 2–56 | 3.68 (3.63–3.73) | 4.4 | MS | 7.7 | S | |
| Chit 3–13 | 6.87 (6.82–6.92) | 2.0 | R | 2.0 | R | |
| Chit 3–30 | 0.93 (0.89–0.96) | 6.3 | S | 8.0 | S | |
| Chit 3–45 | 1.12 (1.08–1.16) | 4.7 | MS | 8.3 | HS | |
| Chit 4–9 | 5.39 (5.24–5.54) | 2.9 | MR | 8.6 | HS | |
| Chit 4–81 | 4.99 (4.89–5.09) | 3.3 | MR | 8.0 | S | |
| Chit 5–65 | 4.29 (4.14–4.44) | 2.9 | MR | 8.3 | HS | |
Values are means of three replicates and the numbers in parentheses represent range of change in endochitinase expression as determined by 2-ΔΔCT method. NTC denotes non-transgenic control plant.
*Refers to red rot rating scale developed by Srinivasan and Bhat (1961), where R = Resistant (0–2), MR = Moderately Resistant (2.1–4), MS = Moderately Susceptible (4.1–6), S = Susceptible (6.1–8), HS = Highly Susceptible (>8).