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(A) Target mutations in alt and modA genes that lead to the abolishment of ADP-ribosylation activity. (B) Design of TE experiment. (C) Evaluation of Cas-mediated T4 DNA TE in vivo in the presence or absence of NgTET/NgTET D234A (two-sided Student’s t-test, *—Psignif < 0.05, **—Psignif < 0.025, ***—Psignif < 0.0125). n = 3 biological replicates.
