Table 1. Summary of phage genome editing efficiency in the presence and absence of NgTET (in green: conditions resulting in positive clones).

Edit Name	Edited gene	Mutagenesis strain (E. coli BL21(DE3)…)	Plaques screened	Mutants identified	Mutation success rate (%)
alt E577A	alt	alt E577A, Cas9_Alt	47	0	0
		NgTET_alt E577A, Cas9_Alt	45	0	0
		alt E577A, Cas12_Alt	38	0	0
		NgTET_alt E577A, Cas12_Alt	44	1	2.6
modA E165A	modA	modA E165A, Cas9_ModA	36	0	0
		NgTET_ modA E165A, Cas9_ModA	36	1	2.8
		modA E165A, Cas12_ModA	35	0	0
		NgTET_ modA E165A, Cas12_ModA	36	2	5.6