FIG. 1.
Candidacidal activity of MUC7 12-mer-l, MUC7 12-mer-d, Hsn5 12-mer, and magainin-II in human whole saliva and saliva supplemented with PIC or EDTA. Panels A, C, E, and G show results for clarified saliva (filter-sterilized saliva), while panels B, D, F, and H show results for unclarified saliva. Each peptide, at a final concentration of 25, 50, or 100 μM, was incubated with 20 μl of C. albicans (DIS isolate at 1 × 105 cells/ml) and 20 μl of clarified or unclarified saliva (SL; hatched bars), saliva containing PIC (SL+PIC; filled bars), or saliva containing EDTA (SL+EDTA; clear bars) at 37°C for 1.5 h. At the end of incubation, the samples were diluted 20-fold and aliquots plated on SDA. The CFU were counted after 24 h of incubation. The loss of C. albicans viability was determined by the equation (1 − CFU of the test group/CFU of the control group) × 100%. The data represent three individual trials, and in each trial the sample was duplicated; the error bars represent standard deviation.