FIG. 4.

Hemolysis caused by the antifungal agents amphotericin B (AMB, ▴), MUC7 12-mer-l (×), MUC7 12-mer-d (⋄), and PIC (▪). Human erythrocytes (final concentration, 0.5%) were incubated for 1 h at 37°C with a twofold dilution series of peptides in PBS or IGP buffer (29). Hemolysis was determined by an absorbance reading of the supernatant at 450 nm and compared to hemolysis achieved with 1% Triton X-100 (reference for 100% hemolysis). Values represent the mean of results from two independent experiments, each of which was done in duplicate.