FIG. 5.
Effect of peptides on viability of (A) HOK-16B cells and (B) KB cells. The toxic effect of the compounds on the cells was determined by calorimetric assay using a CellTiter 96 AQueous One Solution cell proliferation assay (MTS) kit, as described in the text. Briefly, the cells were grown to about 80% confluence, harvested, washed, resuspended in KGM, and dispensed into a 96-well microtiter plate. They were then exposed to different concentrations of peptides or amphotericin B at 37°C and 5% CO2 for 1.5 h. MTS agent was then added to each well, and the cells were further incubated for 4 h. Absorbance at 490 nm was then measured using a microplate reader. MTS reduction is an indication of cell viability. The viability is expressed as relative absorbance (percentage of no-agent control). Data represent the average and standard deviation of results from two independent experiments.