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. Author manuscript; available in PMC: 2024 Sep 16.
Published in final edited form as: Nat Protoc. 2022 Feb 2;17(3):819–846. doi: 10.1038/s41596-021-00667-2

Fig. 3. Comparison of fixation conditions for expanded RPE cells stained by FLARE.

Fig. 3.

Red corresponds to amine stain, green corresponds to carbohydrate stain, and blue corresponds to nuclear stain. (a-d) Fixation with paraformaldehyde and glutaraldehyde generally preserved membrane-bound organelles and other structures while (e-h) fixation with glyoxal showed substantial perturbations. Scale bar (pre-expansion): 3 µm (a-h). Panels a-d were adapted from Mao et al.3 Science Advances. AAAS Publishing Group.