Table 1. Cryo-EM data collection, refinement, and validation statistics.
| CBC-mALYREF2(EMDB EMD-40739)(PDB 8SRR) | CBC(EMDB EMD-40780)(PDB 8SUY) | |
|---|---|---|
| Data collection and processing | ||
| Microscope/camera | Glacios/Falcon 4i | |
| Voltage (kV) | 200 | |
| Electron exposure (e–/Å2) | 52 | |
| Defocus range (μm) | –1.0 to –2.0 | |
| Pixel size (Å) | 0.732 | |
| Box size (pixels) | 288 | |
| Initial particle images (no.) | 1,625,826 | |
| Final particle images (no.) | 241,915 | 78,039 |
| Map resolution (masked, Å) | 3.22 | 3.38 |
| Fourier shell correlation (FSC) threshold | 0.143 | 0.143 |
| Refinement | ||
| Model resolution (masked, Å) | 3.5 | 3.6 |
| FSC threshold | 0.5 | 0.5 |
| Model composition | ||
| Protein residues | 975 | 895 |
| Ligands | 1 | 1 |
| B factors (Å2) | ||
| Protein | 162.9 | 180.1 |
| Ligand | 157.5 | 163.2 |
| r.m.s. deviations | ||
| Bond lengths (Å) | 0.003 | 0.003 |
| Bond angles (°) | 0.417 | 0.413 |
| Validation | ||
| MolProbity score | 1.34 | 1.48 |
| Clashscore | 6.24 | 7.37 |
| Poor rotamers (%) | 0.7 | 0.9 |
| Ramachandran plot | ||
| Favored (%) | 98.0 | 97.6 |
| Allowed (%) | 2.0 | 2.4 |
| Disallowed (%) | 0.0 | 0.0 |