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. 2001 Feb;75(3):1450–1458. doi: 10.1128/JVI.75.3.1450-1458.2001

FIG. 2.

FIG. 2

Construction of HCMV BAC plasmids encoding an SCP-GFP fusion protein (A) and structural analysis of the mutated BAC plasmids (B). (A) Structure of the genomic region of the HCMV BAC plasmids containing the SCP ORF (diagram 1) or the SCP-GFP ORF (diagrams 2 and 3). The arrow indicates the orientation of the SCP ORF. The kanamycin resistance marker (Kan) was excised from BAC plasmid pHB5::SCP-GFP-Kan (diagram 2) by Flp recombinase-mediated site-specific recombination, resulting in BAC plasmid pHB5::SCP-GFP (diagram 3). Fragment sizes are indicated in kilobase pairs below each diagram. (B) DNA of the HCMV BAC plasmids was digested with EcoRV, separated on a 0.7% agarose gel, and stained with ethidium bromide. The sizes of the EcoRV fragments characteristic of parental BAC plasmid pHB5 (lane 1) and mutant BAC plasmids pHB5::SCP-GFP-Kan (lane 2) and pHB5::SCP-GFP (lane 3) are indicated.