Figure 2. T cell phenotype in mice with CALR^del52-MPL or control BM.

(A, B) Scatter plot showing absolute counts of total CD3⁺CD8⁺ T cells (A) and CD3⁺CD8⁺CD69⁺ activated T cells (B) isolated from the spleens of mice injected with BM cells expressing CALR^del52-MPL or control BM. Pooled data from two individual experiments. P values were calculated using unpaired Student’s t-test.

(C-F) Scatter plot showing frequencies of effector memory (CD62L^- CD44^-) T cells (C), effector (CD62L^- CD44⁺) T cells (D), central memory (CD62L⁺ CD44⁺) T cells (E) and naïve (CD62L⁺ CD44^-) T cells (F) in total CD3⁺CD8⁺ viable T cells isolated from the spleens of mice injected with BM cells expressing CALR^del52-MPL or control BM. Pooled data from two individual experiments. P values were calculated using unpaired Student’s t-test.

(G) Representative dot plot showing t-distributed stochastic neighbour embedding (t-SNE) of viable CD3⁺CD8⁺ T cells derived from the spleens of mice injected with BM cells expressing CALR^del52-MPL or control BM. For visualization, previously gated T cell subsets (C-F) are superimposed as indicated.