Figure 2.

Alphavirus replication kinetics on HeLa ΔG3BP cells

(A) HeLa and HeLa ΔG3BP cells were infected with various alphaviruses (0.1 TCID₅₀/cell, n = 2). Supernatant fractions were collected and titrated to determine alphavirus growth kinetics (detection limit 3 log₁₀TCID₅₀/mL).Venezuelan equine encephalitis virus TC-83 (TC-83), bebaru virus (BEBV), and Semliki Forest virus (SFV) demonstrated to replicate independently of G3BP, whereas Barmah Forest virus (BFV), chikungunya virus (CHIKV), getah virus (GETV), Mayaro virus (MAYV), Middelburg virus (MIDV), Ross River virus (RRV), southern elephant seal virus (SESV), Una virus (UNAV), and Whataroa virus (WHAV) required the presence of G3BP for efficient virus replication.