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Tropism and persistence of SAM vaccine in vivo
(A) C57BL/6J mice were injected subcutaneously into the hindfeet with SAM vaccine (SAM-mCherry, 2 × 105 VRPs/foot). (B) One day post injection, only inguinal lymph nodes (ILNs) and feet were positive for SAM vaccine by nsP2 sequence qRT-PCR detection. (C) qRT-PCR illustrated that the majority of SAM was retained in the feet with ≈1/100 disseminating to the draining inguinal lymph nodes. The cut-off for detection by PCR was Ct > 40.
