Figure 5.

In vivo SAM vaccine and GETV co-injection

(A) Schematic of experimental set-up. SAM vaccine encoding mCherry and GETV were co-injected subcutaneously into hindfeet of C57BL/6J, Rag1^−/− and Ifnar^−/− mice. Serum and feet were collected at the indicated days post injection. (B) GETV viremia in the indicated mouse strains (n = 12 or 9 on day 1 and decrease to n = 3 on day 5 for Ifnar^−/− mice, and n = 3 on day 14 for C57BL/6J and Rag1^−/− mice). (C) RNA-seq of feet; counts of reads aligning to the SAM or GETV sequence. (D) Serum samples were passaged a total of four times on HeLa and HeLa ΔG3BP cells. CPE was noted by bright-field inverted microscope with indication of the number of serum samples tested. (E) qRT-PCR of serum samples using primers for SAM and GETV nsP2 sequences.