Figure 4.

CEA-CAR expression in the NK-92 sorted population and in the clones

(A) Flow cytometry analysis of CEA-CAR basal expression in each clone; the red box indicates clones with the lowest CEA-CAR basal expression, selected for subsequent induction experiments against HER2amp cells (two independent experiments). (B) Flow cytometry analysis of CEA-CAR induction in selected clones after co-culture with HER2amp SKBR3; the red box indicates the clones with the highest induction, selected for subsequent induction experiments against all target cells. Bars are SDs (three independent experiments). (C) Flow cytometry plots displaying CEA-CAR induction in NK-92 HER2 synNotch/CEA-CAR after co-culture with HER2 normal and amplified cells (numbers in the square indicate the fraction of CEA-CAR positive cells).