Figure 5.

NK-92.5F clone characterization

(A) Flow cytometry analysis of the major NK/T cell antigen markers on the NK-92 WT and on the NK-92.5F clone. (two independent experiments). (B) Dot plot showing the correlation between NK-92 WT and 5F clone mRNA expression. Black dots = all genes; red dots = NK-specific gene signature. (C) Flow cytometry histograms of CEA-CAR induction after co-culture for different times with HER2amp cells, of either the sorted population (left) or the NK-92.5F clone (right) (three independent experiments). (D) Flow cytometry histograms of CEA-CAR induction and time course suppression after removal of HER2amp target cells, of either the sorted population (left) or the NK-92.5F clone (right). %, percent of cells above the depicted positivity threshold; MFI, mean fluorescence intensity of all cells (three independent experiments).