Figure 6.

NK-92.5F in vitro biologic activity

(A) Specific killing activity of irradiated NK-92.5F against SKBR3, LS180, CRC0080, and CRC0186 cells after 48 h of co-culture at different effector:target ratio. Bars are SD (three independent experiments). Two-way ANOVA p-values: ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001. (B) Degranulation and cytokine release upon co-culture with SKBR3, LS180, CRC0080, and CRC0186 with irradiated effectors. Bars are SDs. Two-way ANOVA p-values: ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001 (three independent experiments for CD107a; four independent experiments for IFN-γ; three independent experiments for granzyme B). (C) Representative confocal microscopy images of CRC0080 organoids grown for 3 days in the presence of different NK-92 effectors, as indicated. Cancer cells were stained with NucBlue (blue signal), and NK-92 cells were stained with PHK26 (red signal). Original magnification ×20; scale bars, 100 μm.