Fig. 3. Specific lysis of GBM cells by Fn14-redirected RevCAR T cells.

U251 Luc and U343 Luc cells expressing luciferase (Luc) were co-cultured with either (A) RevCAR-E5B9 or (B) RevCAR-E7B6 T cells at an effector to target (E:T) cell ratio of 1:2 in the absence or presence of RevTMs (50 nM) for 20 h. Cytotoxic activity was measured using a luminescence-based cytotoxicity assay. C Half-maximal effective concentration (EC₅₀) of Fn14-specific RevTMs was estimated in a co-culture of tumor cells and RevCAR T cells with an E:T ratio of 1:2 (20 h of incubation). A–C Data are shown for three independent T cell donors and represented as mean ± SD (one-way ANOVA with Tukey multiple comparisons test, ****p < 0.0001; comparison to samples without (W/O) RevTM or with irrelevant RevTM).