Figure 3.

Established biofilms of UHW3 and PA14 are not eradicated with honey. (A) UHW3 and PA14 were grown overnight at 37°C to form established biofilms. The biofilms were then exposed to honey concentrations ranging from 2% (w/v) to 64% (w/v) (n = 3). Biofilms were stained with crystal violet and the OD was obtained to determine the concentrations of honey required to eradicate biofilm growth. Colony forming units per milliliter were calculated for (B) UHW3 (n = 3) and (C) PA14 (n = 3) colony forming to determine the viable colonies still present after 24 h incubation at 37°C with honey. Error bars are represented as standard deviation. Significance was determined with a one-way or two way Anova Tukey test. An asterisk indicates significance (p < 0.05).