Figure 4.

Honey scaffolds do not impact the growth of UHW3 and PA14 biofilm co-cultures. UHW3 and PA14 biofilms were established at a ratio of 250:1 in a 24-well plate with honey scaffolds and incubated for 24 h at 37°C. The colony forming units for (A) UHW3 and (B) PA14 were determined by performing a series of 1/10 dilutions and plating each dilution on selective MSA plates or PSA plates, respectively (n = 3). Error bars are represented as standard deviation. White bars with squares represent planktonic CFUs, and shaded bars with triangles represent biofilm CFUs. A two-way anova with Tukey’s multiple comparison was used to determine significance at an alpha level of 0.05. An asterisk indicates significance (p < 0.05).