FIG. 4.
Expression and purification of recombinant TgIMPDH. Transgenic parasite clones stably expressing recombinant TgIMPDHFLAG were selected in mycophenolic acid and xanthine following the electroporation of plasmid into RHΔHX parasites. TgIMPDH was purified by FLAG-based affinity chromatography and eluted from the resin with 3× FLAG peptide. Lysate (L), the unbound fraction (U), and the eluate (E) were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and proteins were visualized by SimplyBlue stain. Molecular mass markers are shown in kilodaltons.