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. 2005 Jun;49(6):2399–2406. doi: 10.1128/AAC.49.6.2399-2406.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 1. — Purification of SAMP H1. (A) HiPrep SP cation-exchange chromatography of SepPac C₁₈-concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of RPC-concentrated active fractions obtained upon HiPrep SP chromatography; (C) final rechromatography on Mono S without urea. Solid lines, absorbance at 280 nm for panel A and absorbance at 214 nm for panels B and C; dotted lines, concentration (conc) of buffer B. The horizontal bars show the fractions that contained antimicrobial activity. mAU, milli-absorbance units.