FIG. 3.

Activation of synthetic SAMP H1. SAMP H1 (50 μg) was applied to a Mono S column with (black lines) and without (grey lines) addition of PPIase. Solid lines, number of μg of peptide activated; dotted lines, relative activity. The amount of activated peptide was measured by determination of the A₂₁₄ after separation of the active and inactive forms by Resource RPC. Antimicrobial activity was measured by the radial diffusion assay, with 100% relative activity being equal to the activity obtained without PPIase at 0 h.