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. 2005 Jun;49(6):2399–2406. doi: 10.1128/AAC.49.6.2399-2406.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 4. — Chromatographic properties of the different forms of SAMP H1. Chromatographic properties of natural (A and D), inactive synthetic (B and E), and activated synthetic (C and F) SAMP H1 on Mono S (A to C) and Superdex Peptide (D to F) columns. The black horizontal bars in panels A to C show the fractions that contained antimicrobial activity, while the dotted bar in panel B shows the fractions that contained inactive SAMP H1. The void volume (V₀) and total volume (V_T) of the Superdex Peptide column are indicated by the arrows in panel D. Solid lines, absorbance at 214 nm; dotted lines, concentration of buffer B; mAU, milli-absorption units.