FIG. 2.

Detection of pncA mutations by PCR fragments A through D. Isolates were scanned for mutations associated with PZA resistance in pncA using DGGE PCR products A, B, C, and D. Mutations were detected only within a single PCR product, and this allowed for the localization of mutations within the pncA open reading frame. Isolate (mutation): 1 (G17S, GGC→AGC); 2 (I5-frameshift, ATC→ATG TC…); 3 (Y103stop, TAC→TAG); 4 (V93L, GTG→CTG); 5 (T160TPE, ACA GCG→ACA CCT GAA GCG); 6 (V180F, GTC→TTC).