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. 2005 Jun;49(6):2210–2217. doi: 10.1128/AAC.49.6.2210-2217.2005

FIG. 4.

FIG. 4.

Detection of pncA mutations by fragments B to D. Isolates in lanes 1 to 27 were scanned for mutations with PCR products B, C, and D as indicated. The presence of multiple bands on the denaturing gel indicates that DNA alterations were detected within the fragment. See Table 2 for details of mutations and altered codons. Mutations were detected only within one PCR fragment except where indicated. Lane(s) (altered amino acid): 1 (H71Y); 2 (S65S); 3 (H57D); 4 (H71E); 5 (G75G); 6 (P54L; also detected within fragment E); 7 (S67P); 8 (T76P); 9, 18, and 27 (wild type, H37Rv); 10 (Y103H); 11 (Y103stop); 12 (V93L); 13 (K96T); 14 (G97S); 15 (E91stop); 16 (IS6110 insertion at codon 114); 17 (V125F; also detected within fragment D, not shown); 19 (V180F); 20 (T160TPE); 21 (V139A); 22 (T160P); 23 (A134V); 24 (R154G); 25 (M175V); 26 (A134V).