TABLE 2.

Sequenced pncA mutations

Specimen no.a	Codonb	Base changec	Amino acid changed	DGGE fragment(s)e	Fig:lanef
2778	4	TTG→TCG	Leu→Ser	A	3:1
4067	5	ATC→A^TG TC...	Ile→Met + fs	A	2:2, 3:2
5242	12	GAC→GCC	Asp→Ala	A, E	3:3
2381	17	GGC→AGC	Gly→Ser	A, E	2:1, 3:4
3579	27	CTG→CCG	Leu→Pro	(A), E	3:5
2609	35	CTG→CCG	Leu→Pro	(A), E	3:6
2886	41	TAC→TAG	Tyr→Stop	(A), E	3:7
3772	47	ACC→GCC	Thr→Ala	(A), E	3:8
1908	49	GAC→GCC	Asp→Ala	(A), E	3:9
	75	GGT→GGC	Gly→Gly	B	4:5
1417	54	CCG→CTG	Pro→Leu	E, B	3:10, 4:6
5745	57	CAC→GAC	His→Asp	B	4:3
4508i	65	TCC→TCT	Ser→Ser	B	4:2
4862	67	TCG→CCG	Ser→Pro	B	4:7
2753	71	CAT→TAT	His→Tyr	B	4:1
3377	71	CAT→GAT	His→Asp	B	4:4
5482	76	ACT→CCT	Thr→Pro	B	4:8
2091	91	GAG→TAG	Glu→stop	C	4:15
4086	93	GTG→CTG	Val→Leu	C	2:4, 4:12
4074	96	AAG→ACG	Lys→Thr	C	4:13
820	97	GGT→AGT	Gly→Ser	C	4:14
2319	103	TAC→TAG	Tyr→stop	C	2:3, 4:11
3455	103	TAC→cAC	Tyr→His	C	4:10
5171	114	114̂IS6110	fs	C	4:16
570	125	GTC→TTC	Val→Phe	C, Dg	4:17
1902	134	GCC→GTC	Ala→Val	D	4:23
5330	134	GCC→GTC	Ala→Val	D	4:26
1419	139	GTG→GCG	Val→Ala	D	4:21
1969	154	AGG→GGG	Arg→Gly	D	4:24
4694	160-161	ACA GCG→AĈA CCT GAA GCG	Thr Ala→ThrPro Glu Ala	D	2:5, 4:20
5387	160	ACA→CCA	Thr→Pro	D	4:22
4259	175	ATG→GTG	Met→Val	D	4:25
4547	180	GTC→TTC	Val→Phe	D	2:6, 4:19
4720	None	None	None	None

^aAll isolates are resistant to pyrazinamide except 4508; isolate 5745 is M. bovis.

^bCodons of pncA (Rv2043c) open reading frame.

^cBase change of altered codon with altered nucleotide(s) underlined; ^, insertion; lowercase letter, minor nucleotide mixed with wild type as determined by DNA sequence.

^dAmino acid alteration of mutation is indicated; fs, frameshift; stop, nonsense mutation; ^, insertion.

^eDNA alteration was identified within DGGE fragments A to E as indicated; (A), mutation is within fragment A but was not detected.

^fFig:lane, figure and lane in which the denaturation profile of the mutation is shown.

^gDGGE profile is not shown.