TABLE 1.

In vitro activities of CAS and AMB, alone and in combination, determined by the checkerboard method, against two clinical isolates of C. glabrata^a

Isolate no.	Reading criterionb	Median (range) CAS MIC (μg/ml) tested:		Median (range), AMB MIC (μg/ml) tested:		Type of interaction by:
		Alone	In combination	Alone	In combination	FIC rangec	Definition
4205	IC50	1.0 (1.0-2.0)	0.25 (0.125-0.5)	1.0 (1.0-2.0)	1.0 (1.0)	1.06-1.25	Indifference
	IC90	4.0 (4.0)	1.0 (0.5-1.0)	1.0 (1.0-2.0)	1.0 (1.0)	0.75-1.12	Indifference
4293	IC50	1.0 (1.0)	0.25 (0.125-0.5)	1.0 (1.0-2.0)	0.5 (0.25-1.0)	0.625-1.06	Indifference
	IC90	4.0 (2.0->4.0)	1.0 (0.25-2.0)	2.0 (2.0)	1.0 (0.125-2.0)	1.03-1.09	Indifference

^aEach isolate was tested in quintuplicate.

^bReadings were performed spectrophotometrically with an automatic plate reader; two endpoints were considered: the first concentration of the antifungal agent tested alone or in combination which the turbidity in the well was either 50% (inhibitory concentration [IC₅₀]) or 90% (IC₉₀) less than that in the control well.

^cFIC index. The interaction was defined as synergistic if the FIC index was less than or equal to 0.50, indifferent if the FIC index was greater than 0.50 and less than or equal to 4.0, and antagonistic if the FIC index was greater than 4.0 (5).