TABLE 4.
Detection of proviral sequence in PBMC of the long-term monitored SIVmac251-challenged monkeys
|
nef detection after a:
| |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Animal | Day 0 (Δnef) | 0.5 mo
|
1 mo
|
2 mo
|
3 mo
|
7 mo
|
12 mo
|
32 mo
|
56 mo
|
62 mo
|
|||||||||||
| Δnef | Wt | Δnef | Wt | Δnef | Wt | Δnef | Wt | Δnef | Wt | Δnef | Wt | Δnef | Wt | Δnef | Wt | Δnef | Wt | ||||
| 49848 | + | + | − | − | − | + | − | − | − | + | − | + | − | + | − | − | − | + | − | ||
| 49851 | + | − | − | + | + | + | + | + | + | + | − | − | − | + | − | HYB | HYB | ||||
a
Total DNA corresponding to 2 × 105 cells was subjected to nested PCR amplifying a nef gene portion. Δnef, nef of the vaccine virus; Wt, nef of the challenge virus; HYB, nef of intermediate size; +, positive PCR amplification; −, negative PCR amplification.