Fig. 1.

Microextrusion bioprinting reproducibly produces millimeter-sized breast cancer models with distinct cancer and stromal areas.

a. Bioink formulation schematic. b. Schematic representation of the 3-step bioprinting process. c. Bioprinted breast cancer model (BpBCM) design. d. BpBCM epifluorescence imaging 4h post-printing with MCF-7 GFP+ (green) in the center and HUVEC mKate+ (red) in the periphery (left). Scale bar: 1000 μm. Cancer center and stromal periphery contour detection (right). e,f. Cancer center and stromal periphery contour distribution (e, scale bar: 1000 μm), and quantifications of contour diameter and roundness (f) (N = 3, n = 62). Shown as mean ± s.d.