Fig. 3.

BpBCM stromal compartment favors overtime maturation of cancer cells and microvascular structures

a. Representative ki67 immunostaining (gray), and stained nuclei using DAPI (blue), of BpBCM models printed with MCF-7 GFP+ (green) in co-culture conditions. Scale bar: 100 μm. b,c. Spheroid volume (b) and ki67 spheroid positivity (c) on day 4 and 7 in the different culture conditions (N = 3, n = 10–13). d. Spheroid, either positive or negative for ki67, volume distribution (log scale). e. Representative 3D acquisition maximum intensity projection of the BpBCM stroma after 7 days of culture. BpBCM models were printed with HUVEC mKate+ (red) in the stromal periphery in different co-culture conditions. Scale bar: 100 μm. f. 3D network analysis and quantification of total vessel length, volume and number of branchpoints (N = 3, n = 10–14). g. Immunostaining of triCAF model for VE-Cadherin (yellow), HUVEC mKate+ (red), nuclei (blue), full model imaging (left), and close up on one representative vessel (right). Scale bars: 1000 μm (left), 50 μm (right). All data represent mean ± s.d. Each dot represents a single spheroid (d). P-values were calculated with Kruskal-Wallis test corrected by Dunn's multiple comparison test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.