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. 2024 Aug 1;43(18):4092–4109. doi: 10.1038/s44318-024-00181-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) Expression profiles of the three M. polymorpha CYP73 paralogs in various tissues. Data are derived from the CoNekT database (https://evorepro.sbs.ntu.edu.sg; Proost and Mutwil, 2018), except sporophytes for which data derive from the MarpolBase database (https://marchantia.info/mbex/). (B) RT-qPCR expression analysis of the three MpCYP73 genes in 3-week-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. Results are the mean ± SEM of four biological replicates. (C) Representative pictures of 3-week-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. (D, E) Quantification of thallus area (D) and gemmae cup number (E) in 3-week-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. Results are the mean (pink trait) of measurements made on 29–32 plants. WT versus mutant one-way ANOVA adjusted P-value is displayed. (F) Representative UHPLC-UV chromatograms of 1-month-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 crude extracts. (G) Relative quantification of shikimate phenolic esters in 1-month-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. C5S, caffeoyl-5-O-shikimate; pC5S, p-coumaroyl-5-O-shikimate. (H) Quantification of hydroxycinnamic acids from saponified extracts of 1-month-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. (I) Compositional analysis of the cuticular polymer in 1-month-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. (J) Total amount of cuticular monomers in 1-month-old wild-type, Mpcyp73a1-1 and Mpcyp73a1-2 plants. Results in panels (G), (H), (I), and (J) are the mean ± SEM of four independent biological replicates for each genotype. WT versus mutant unpaired t test adjusted P-value: *P < 0.05; **P < 0.01; ***P < 0.001. nd, not detected. Source data are available online for this figure.